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13. V ml of a solution containing picramic and picric acids were placed in a separatory funnel, the necessary reagents were added, including a solution of cationic red-violet dye, which allows picric acid to be extracted into a benzene layer. When extracting 2.00 ml of benzene and measuring the optical density of the organic extract, Ax was obtained. Calculate the concentration of picric acid in the solution (mg/l) using the following data:
Option 1 2 3
V, ml 10.00 15.00 20.00
Ax 0.480 0.660 0.840
To plot a calibration graph, use the data obtained during extraction under the same conditions of a solution containing 1.10 μg/ml of picric acid:
Vst 1.50 3.50 5.50 7.50 9.50
Ast 0.180 0.360 0.620 0.780 1.00
Solution for option 1
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