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816. Determine the Michielis constant and the reversal number of the a-chymotrypsin enzyme in the reaction of N-acetyl-L-valine methyl ester hydrolysis using the following data obtained at an initial enzyme concentration of 38 µmol/l at 25 C in a 0.1 mol/l KCl solution ( [S]0- initial concentration of the substrate, v0- initial rate of hydrolysis):
[S]0, mol/l 0.200 0.124 0.091 0.071 0.060
v0*106, mol/(l*s) 4.57 3.83 3.31 2.93 2.67
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